Journal: Scientific Reports

Article Title: Woodchuck and deer Hepatitis Delta-like agents show distinct innate immune activation and IFN-resistance compared to human Hepatitis D Virus

doi: 10.1038/s41598-026-45998-w

Figure Lengend Snippet: Effect of interferon treatment on cell division-mediated spread of WoDV and DeDV in HuH7 NTCP cells. (A) Experimental design: HuH7 NTCP cells were infected with HDV, WoDV, or DeDV pseudoparticles and passaged at day 6 pi (1:100 dilution). During clonal expansion, cells were treated with IFN-α2A (200 IU/mL). (B) Cells were fixed at confluence and stained with FD3A7 antibody for DAg. Representative fields are shown at low and higher magnification. Scale bar: 200 μm. (C) Clusters of DAg-positive cells were quantified using CellProfiler software; data represent mean ± SD from three independent experiments. (D) Viral RNA levels were assessed by Northern blotting with DIG-labeled probes specific for HDV, WoDV, or DeDV. Representative blot shown (uncropped version in Fig. S8). (E) IF co-staining for DAg (FD3A7 antibody) and Mx1 (Santa Cruz, Cat# sc-271527) was performed. Nuclei were counterstained with Hoechst 33342. Representative images from three experiments are shown. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Student’s t test).

Article Snippet: The cells were treated with 200 IU/mL IFN-α2A (human) (PBL Assay science, Cat:11100-1), 500 IU/mL IFN-αA/D (pan-species IFN) (Sigma, Cat:14401) or 0.1 μg polyinosinic-polycytidylic acid (Poly I: C).

Techniques: Infection, Staining, Software, Northern Blot, Labeling

Journal: Scientific Reports

Article Title: Woodchuck and deer Hepatitis Delta-like agents show distinct innate immune activation and IFN-resistance compared to human Hepatitis D Virus

doi: 10.1038/s41598-026-45998-w

Figure Lengend Snippet: Effect of L-HDAg on interferon sensitivity of HDV. (A , D) HepaRG NTCP (A) and HuH7 NTCP (D) cells were infected with wild-type (WT) HDV or an L-HDAg-deficient mutant (HDV-ΔL) . (B , E) At day 6 pi, infection rates were determined by IF staining for DAg (FD3A7 antibody). (C , F) Following high-dilution passage (1:50 for HepaRG NTCP , 1:100 for HuH7 NTCP ), cells were either untreated or treated with Ruxolitinib (5 µM) or IFN-α2A (200 IU/mL) and analyzed at confluence for CDMS by IF. Nuclei were counterstained with Hoechst 33342. Representative images from three independent experiments are shown. Scale bar: 200 μm.

Article Snippet: The cells were treated with 200 IU/mL IFN-α2A (human) (PBL Assay science, Cat:11100-1), 500 IU/mL IFN-αA/D (pan-species IFN) (Sigma, Cat:14401) or 0.1 μg polyinosinic-polycytidylic acid (Poly I: C).

Techniques: Infection, Mutagenesis, Staining

Journal: EMBO Reports

Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons

doi: 10.1038/s44319-026-00750-3

Figure Lengend Snippet: ( A ) Overview of the type I IFN system. ( B ) Schematic representation of the mass cytometry workflow to quantify protein phosphorylation in response to type I IFN-treatment. ( C ) Median expression of pSTAT1, pSTAT3, pSTAT4, pSTAT5 and pSTAT6 in PBMCs treated with increasing concentrations of IFN-α2a for 15 min. ( D ) Depiction of the data shown in ( C ) as a heatmap. ( E ) Violin plots showing expression of each pSTAT in the indicated cell types in response to increasing concentrations of IFN-α2a. The number of cells analysed is shown in parentheses. Violin plots for all cell types are shown in Appendix Fig. . Data are from one experiment with one donor. See also Appendix Figs. and and Datasets and .

Article Snippet: IFN-α2a , PBL Assay Science , Cat# 11100-1.

Techniques: Mass Cytometry, Phospho-proteomics, Expressing